盐胁迫下接种丛枝菌根真菌(AMF)对小叶锦鸡儿的生长及总黄酮含量的影响
Effect of Arbuscular Mycorrhizal Fungi on Growth and the Total Flavonoids of Caragana microphylla Lam. under Salt Stress
摘要:
本文利用盆栽试验，研究不同NaCl盐浓度条件下，接种摩西球囊霉(Glomus mosseae)对小叶锦鸡儿(Caragana microphylla Lam.)生物量及总黄酮含量的影响。结果显示：1) 在正常生长条件下，与不接种(对照)相比，接种G. mosseae小叶锦鸡儿地上部干重、地下部干重和总干重分别增加了17.9%、29.4%和20.0%；叶、茎和根中总黄酮含量分别提高了9.0%、0.9%和11.7%，其中，除茎中的总黄酮差异性不显著外，其余指标与对照差异均达到显著性水平(P < 0.05)。2) NaCl盐胁迫下，小叶锦鸡儿的生长受到明显的抑制，其生物量和总黄酮含量显著降低，并随盐浓度的增加而加剧。3) 盐胁迫下接种G. mosseae显著促进了小叶锦鸡儿的生长，植物干重和总黄酮含量均比未接菌植株高。同时，NaCl盐胁迫也影响小叶锦鸡儿的菌根侵染率和菌根依赖性。盐处理和AMF对生物量干重、叶和根中总黄酮含量的交互作用显著(P < 0.05)。表明AMF不仅能提高小叶锦鸡儿在盐碱地中的生长能力并减轻盐胁迫对植物造成的产量损失，同时也增加了它的黄酮含量，对于推动AMF在盐碱地区植物资源生产与开发利用中具有重要的意义。
A pot experiment was conducted to test the effect of Glomus mosseae on the growth and the total flavonoids of Caragana microphylla Lam. under different NaCl salt treatments. The result indicated that: 1) The inoculation of G. mosseae promoted the growth of C. microphylla under natural growed conditions. The dry weight of the overground part, underground part and the total were increased by 17.9%, 29.4%, 20.0% respectively and the flavonoids content in leaves, stems and roots were increased by 9.0%, 0.9%, 11.7% respectively than non-inoculated plants. Among them, they have a significant difference (P < 0.05)in addition to the total flavonoids content of stems compare to non- nioculated. 2) The biomass and total flavonoids content of C. microphylla significantly reduced under NaCl Stress, and remarkably aggravated with the increasing of the salt stress degree. 3) AMF futher promoted the growth of C. microphylla under salt-stress. The total dry weight and the flavonoids content were higher in inoculated plant than non- inoculated plants. The mycorrhizal infection rate and mycorrhizal dependence of C. microphylla were also affected by NaCl. NaCl and AMF have significant (P < 0.05) interaction effect on the dry weight and the flavonoids content in leaves and roots. AMF not only can improve the growth ability but also can reduce the loss of plant production caused by salt-stress and increase its flavonoids content. It plays an important role to promote the development and utilization of the regional plant resources in saline.
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