粒毛盘菌多酚理化特征及其生物活性研究
Physicochemical Characteristics and Biological Activities of Polyphenols from Lachnum
摘要:
本文作者提取并纯化了粒毛盘菌YMU50胞外多酚(LTEP），研究了其理化特征并测定其抗氧化活性及抑菌活性。紫外光谱显示其在270 nm和340 nm处有最大吸收峰，表现出典型的酚类特征；红外光谱显示其在3354.360 cm-1和1048.762 cm-1处含有酚类的特征吸收峰。抗氧化试验表明，当LTEP浓度为2 mg/mL时，对、DPPH、·OH和的清除率分别为76.43%、72.7%、55.56%、59.4%；抑菌试验显示，LTEP对金黄色葡萄球菌、酿酒酵母、大肠杆菌、伤寒沙门氏菌均具有明显抑制作用，四种菌的最低抑菌浓度(MIC)分别为0.05、0.1、0.1、0.2 mg/mL，结果表明LTEP具有较强的抗氧化活性和抑菌活性。
Lachnum YMU50 total extracellular polyphenols (LTEP) were extracted and purified. The physicochemical properties, antioxidant and antibacterial activities of LTEP were investigated. UV spectroscopy analysis indicated that LTEP had typical characteristics absorption peaks of phenolic at 270 nm and 340 nm. IR spectroscopy displayed that LTEP had characteristics absorption peaks of phenolic at 3354.360 cm-1and 1048.762 cm-1. Antioxidant trials showed that the scavenging effect of LTEP on , DPPH, ·OH and were 76.43%, 72.70%, 55.56% and 59.40% respectively, at the 2 mg/mL concentration of LTEP. LTEP also showed a significantly antibacterial activity against Staphylococcus aureus, S. cerevisiae, E. coli, and Salmonella typhi. The MIC (minimum inhibitory concentration) of these four strains was 0.05, 0.1, 0.1 and 0.2 mg/mL, respectively. The results revealed that LTEP had strong antioxidant and antibacterial activities.
参考文献
|
[1]
|
Zhao, Y.X., Miao, K.J., Zhang, M.M., et al. (2009) Effects of nitric oxide on production of antioxidant phenolic compounds in Phaeoporus obliquus. Mycosystema, 28, 750-754.
|
|
[2]
|
钱梅双, 吴春艳, 纪静等. (2013) 粒毛盘菌YMU50多酚发酵与纯化初步研究. 合肥工业大学学报(自然科学版), 36, 1115- 1121.
|
|
[3]
|
程鑫颖, 包海鹰, 丁燕等 (2011) 瓦宁木层孔菌中多酚和黄酮类成分分离及清除自由基活性的研究. 菌物学报, 30, 281- 287.
|
|
[4]
|
陈向东, 刘晓雯, 吴梧桐 (2005) 灰树花多酚的提取和活性研究. 食品与生物技术学报, 24, 26-30.
|
|
[5]
|
Kang, N.J., Shin, S.H., Lee, H.J., et al. (2011) Polyphenols as small molecular inhibitors of signaling cascades in carcinogene- sis. Pharmacology & Therapeutics, 130, 310-324.
|
|
[6]
|
Terahara, M., Nishide, S. and Kaneko, T. (2002) Preventive effect of lactobacillus delbrueckii subsp bulgaricus on the oxidation of LDL. Bioscience Biotechnol and Biochemistry, 64, 1868-1873.
|
|
[7]
|
Ye, M., Qiu, T., Peng, W., et al. (2011) Purification, characteri- zation and hypoglycemic activity of extracellular polysaccha- rides from Lachnum calycu-liforme. Carbohydrate Polymers, 86, 285-290.
|
|
[8]
|
Ye, M., Wang, Y., Qian, M.S., et al. (2011) Preparation and properties of the melanin from Lachnum singerianum. Interna- tional Journal of Basic & Applied Sciences, 11, 51-58.
|
|
[9]
|
叶明, 朱立 (2008) 辛格粒毛盘菌胞内多酚提取及其抗氧化性研究. 食品科学, 8, 249-252.
|
|
[10]
|
黄建林, 张展霞 (2004) GC-MS分析番石榴叶乙醇提取物的化学成分. 中山大学学报(自然科学版), 43, 117-120.
|
|
[11]
|
Lim, Y.Y. and Murtijaya, J. (2007) Antioxidant properties of Phyllanthus amarus extracts as affected by different drying methods. LWT-Food Science and Technology, 40, 1664-1669.
|
|
[12]
|
Dastmalchi, K., Dorman, D.H.J., Oinonen, P.P., et al. (2007) Chemical composition and in vitro antioxidative activity of a lemon balm (Melissa officinalis L.) extract. LWT-Food Science and Technology, 40, 1664-1669.
|
|
[13]
|
Lee, Y.L., Yen, M.T., Mau, J.L. (2007) Antioxidant properties of various extracts from Hypsizigus marmoreus. Food Chemistry, 104, 1-9.
|
|
[14]
|
Tsai, S.Y., Huang, S.J. and Mau, J.L. (2006) Antioxidant proper- ties of hot water extracts from Agrocybe cylindracea. Food Che- mistry, 98, 670-677.
|
|
[15]
|
陈留勇, 孟宪军, 贾薇等 (2004) 黄桃水溶性多糖的抗肿瘤作用及清除自由基、提高免疫活性研究. 食品科学, 25, 167- 170.
|
|
[16]
|
谢祥茂, 丁小雯, 陈俊琴 (2001) 金樱子提取液对NO2−清除作用的体外实验研究. 食品科学, 22, 30-33.
|
|
[17]
|
李建慧, 马会勤, 陈尚武 (2008) 葡萄多酚抑菌效果的研究. 中国食品学报, 8, 100-106.
|
|
[18]
|
郭炳莹, 程启坤 (1991) 茶汤组分与金属离子的络合性能. 茶叶科学, 11, 139-144.
|
|
[19]
|
贝玉祥, 郭英, 何超等 (2009) 诃子多酚的提取分离结构鉴定及抗氧化活性的研究. 食品研究与开发, 3, 6-9.
|
|
[20]
|
Shriner, R.L. (2007) 有机化合物系统鉴定手册: 第八版. 化学工业出版社, 北京.
|
|
[21]
|
Yen, G.C., Duh, P.D. and Tsai, H.L. (2002) Antioxidant and pro-oxidant properties of ascorbic acid and gallic acid. Food Chemistry, 79, 307-313.
|
|
[22]
|
Kim, D.O., Lee, K.W., Lee, H.J., et al. (2002) Vitamin C equiva- lent antioxidant capacity (VCEAC) of phenolic phytochemicals. Journal of Agricultural and Food Chemistry, 50, 3713-3717.
|
|
[23]
|
陈琪, 王伯初, 唐春红等 (2003) 黄酮类化合物抗氧化性与其构效的关系. 重庆大学学报, 26, 48-51.
|
|
[24]
|
吴春, 陈林林, 李俊生 (2012) 黑大豆种皮花色苷对亚硝胺体内外合成的阻断作用. 中国粮油学报, 27, 25-27.
|
|
[25]
|
严守雷, 王清章, 彭光华等 (2006) 莲藕多酚对微生物抑制作用的研究. 食品研究与开发, 27, 148-151.
|